The accumulation as peptidyl-transfer RNA of isoaccepting transfer RNA families in Escherichia coli with temperature-sensitive peptidyl-transfer RNA hydrolase.
نویسندگان
چکیده
منابع مشابه
Covalent attachment of a peptidyl-transfer RNA analog to the 50S subunit of Escherichia coli ribosomes.
The peptidyl-tRNA analog, N-bromo-acetyl-Phenylalanyl-tRNA(Phe) has been prepared. Its binding to the 70S ribosome of E. coli is totally dependent upon polyuridylic acid. The analog becomes covalently attached to the 50S particle. It is associated with only one protein fraction after polyacrylamide-gel separation of total 50S proteins. The analog also reacts with 23S ribosomal RNA or a protein ...
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Growing cultures of mutant Escherichia coli with temperature-senstive peptidyl-tRNA hydrolase were shifted to nonpermissive 4o degrees. There followed a roughly linear increase in a fraction of isolated tRNA (over 50% after 20 min) whose amino acid-accepting activity was masked until treatment with active peptidyl-tRNA hydrolase. The ionophoretic mobility of amino acid label associated with thi...
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It has been possible to demonstrate the complete absence of either charged tRNA(Glu) or charged tRNA(Val) at 42 degrees by the use of two stringent strains of E. coli, one temperature-sensitive for glutamyl-tRNA synthetase and the other temperature-sensitive for valyl-tRNA synthetase. In both strains, stable RNA synthesis ceases, and guanosine tetraphosphate accumulates upon incubation at the n...
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A defective varphi80 transducing phage has been isolated that carries a small section of the E. coli chromosome from the region near argH (77 min). This phage contains three E. coli tRNA genes, termed glyTsu(+) (36), tyrT, and thrT. These genes specify the structures of tRNA(2) (Gly) (su(+) (36)) tRNA(2) (Tyr), and tRNA(3) (Thr), respectively. Induction of a single lysogen bearing this phage br...
متن کاملStudies on genetically altered transfer RNA species in Escherichia coli.
The study of tRNA molecules altered by a mutational event can lead to information in 3 important areas: (a) structure-function relationships in tRNA biochemistry; (ft) the genetics of tRNA; and (c) the physiological role of tRNA-isoaccepting species. For example, the elucidation of the structural alteration in a mutant tRNA species which reacts appreciably more slowly than normal tRNA in the en...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1978
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)37991-7